Dr Joanna Denbigh
|Room 101, Peel Building, University of Salford, Salford, UK, M5 4WT.|
|0161 295 8152|
My research in the bioanalytical field is multidisciplinary and broadly covers pharmaceutical and biomedical applications employing powerful analytical techniques of mass spectrometry (LC-MS, GC-MS, SIMS, MALDI and DESI) and spectroscopy (FTIR, ATR, Synchrotron-FTIR and Raman microspectroscopy) to probe changes induced in cells as a result of abiotic stress. One key angle is to investigate the mechanistic action of novel drug therapies at a single cell level. I am also interested in studying biochemical changes as a result of drug resistance, hypoxia and toxicity in both cells and tissue. Characterising changes in the lipidome and metabolome of mammalian cells as a consequence of pharmacological induced stress affords new insight into biochemical processes associated with disease progression and the treatment thereof. Many diseases are linked with abnormal lipid metabolism. The chemical profiling of diseased and stressed biological systems through lipidomics and metabolomics is a powerful approach to understanding and ultimately controlling biological function. Through access to Synchrotron beam time in Australia and the UK, I have been able to study changes in living cells when treated with drug compounds and deduce information regarding drug uptake and mode of action. Clinical translation is a key aspect of my research and as a member of the Raman4Clinics network and the Clinical Infrared and Raman Spectroscopy for diagnosis network (CLIRSPEC), I work collaboratively with partners in academia and industry to facilitate progress in this field. I have maintained strong industrial links over the years and enjoy working in partnership with industry collaborators where possible. Memberships: Chair of the Society for Applied Spectroscopy UK branch Member of the British Association for Cancer Research Member of The Royal Society of Chemistry (MRSC) Member of the Metabolomics Society Member of the British Mass Spectrometry Society Member of the Infrared and Raman Discussion Group Honorary Researcher, The University of Manchester
BBSRC funded PhD in Biological Chemistry working in the groups of Dr Nick Lockyer and Professor Roy Goodacre. My project entitled "Lipidomic and Metabolomic Analysis of Biological Response Mechanisms in Cancer Cells" employed a multi-dimensional bioanalytical approach utilizing Secondary Ion Mass Spectrometry (SIMS), Raman Spectroscopy, Infrared Spectroscopy and LC-MS to study biological changes in cells as a response to drug treatment. My work primarily focused on understanding the metabolite and lipid changes of acute myeloid leukaemia cells when treated with a novel drug therapy. Visiting Researcher: Following a successful international peer-reviewed application for funded Australian Synchrotron beam time that I wrote, I was invited to work at Monash University, Australia, in the group of Associate Professor Wood (Feb-March 2015). In conjunction with my own research, I contributed presentations, led discussions in group meetings and ran a series of training sessions in cell culture techniques for University and Synchrotron staff. I was awarded a CLIRSPEC grant to facilitate this research. Research Presentations: Oral presentation at BMSS 2016, UK and EuCheMS 2014, Turkey. Poster presentations at SPEC 2014, Poland; BMSS 2014, UK; SciX 2013, USA; Metabolomics 2013, UK and SIMS Europe 2012, Germany. Prizes: I was awarded two prizes at SciX 2013: The Society for Applied Spectroscopy (SAS) and Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) poster awards for my poster presentation entitled ¿ToF-SIMS and Raman as tools for probing lipid saturation in acute myeloid leukaemia cells treated with a novel combination therapy¿. I was awarded 2nd prize for School of Chemistry final year PhD presentations, June 2015
Awarded a MRSEC Scholarship from the University of Nebraska, USA (June-August 2003). Research project in the Department of Chemistry for Dr J. Belot into inorganic solid-state synthesis of lanthanide nitrides. Awarded The Royal Society of Chemistry 2003 Prize in Analytical Chemistry for most promising student in analytical chemistry 3rd Year Industrial Placement Year, Sep 2003-Aug 2004, Pfizer Ltd, Sandwich In the Pharmacokinetics, Dynamics and Metabolism department, I carried out a research project into the identification and quantitation of radiolabelled low-level drug metabolites. I worked with a variety of biological matrices across all areas of pharmacokinetics and drug metabolism and gained extensive instrumental experience, particularly in HPLC-MS/MS and radiochemical detection.
Refereed Journal Articles