SEEK: Salford Environment for Expertise and Knowledge

Journal Article (Refereed)
January 2010

Fusion-activated Ca2+ entry: an żactive zoneż of elevated Ca2+ during the postfusion stage of lamellar body exocytosis in rat type II pneumocytes

Miklavc, P & Frick, M & Wittekindt, O & Haller, T & Dietl, P 2010, 'Fusion-activated Ca2+ entry: an żactive zoneż of elevated Ca2+ during the postfusion stage of lamellar body exocytosis in rat type II pneumocytes', PLoS ONE, 5(6), pp.1-10.

Abstract

BACKGROUND:
Ca(2+) is essential for vesicle fusion with the plasma membrane in virtually all types of regulated exocytoses. However, in contrast to the well-known effects of a high cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) in the prefusion phase, the occurrence and significance of Ca(2+) signals in the postfusion phase have not been described before.


METHODOLOGY/PRINCIPAL FINDINGS:
We studied isolated rat alveolar type II cells using previously developed imaging techniques. These cells release pulmonary surfactant, a complex of lipids and proteins, from secretory vesicles (lamellar bodies) in an exceptionally slow, Ca(2+)- and actin-dependent process. Measurements of fusion pore formation by darkfield scattered light intensity decrease or FM 1-43 fluorescence intensity increase were combined with analysis of [Ca(2+)](c) by ratiometric Fura-2 or Fluo-4 fluorescence measurements. We found that the majority of single lamellar body fusion events were followed by a transient (t(1/2) of decay = 3.2 s) rise of localized [Ca(2+)](c) originating at the site of lamellar body fusion. [Ca(2+)](c) increase followed with a delay of approximately 0.2-0.5 s (method-dependent) and in the majority of cases this signal propagated throughout the cell (at approximately 10 microm/s). Removal of Ca(2+) from, or addition of Ni(2+) to the extracellular solution, strongly inhibited these [Ca(2+)](c) transients, whereas Ca(2+) store depletion with thapsigargin had no effect. Actin-GFP fluorescence around fused LBs increased several seconds after the rise of [Ca(2+)](c). Both effects were reduced by the non-specific Ca(2+) channel blocker SKF96365.


CONCLUSIONS/SIGNIFICANCE:
Fusion-activated Ca(2+)entry (FACE) is a new mechanism that leads to [Ca(2+)](c) transients at the site of vesicle fusion. Substantial evidence from this and previous studies indicates that fusion-activated Ca(2+) entry enhances localized surfactant release from type II cells, but it may also play a role for compensatory endocytosis and other cellular functions.

Authors

SEEK Members

External Authors

T Haller

P Dietl

M Frick

OH Wittekindt

Publication Details

Journal Name
PLoS ONE

Volume
5(6)

Pagination
1-10.