SEEK: Salford Environment for Expertise and Knowledge
Dr David Greensmith
Dr David Greensmith
postal addressG35, Peel Building .
telephone0161 2952170
Key Memberships
Full Member of Research Centre: Biomedical Research Centre
From September 2015 to present.
From December 2014 to August 2015.
Profile Summary


I graduated from The University of Salford in 2005 having completed a BSc Biological Sciences. I went on to do my PhD with Dr Mahesh Nirmalan and Professor David Eisner in the School of Medicine, University of Manchester. The main focus of my work was to investigate the effects of reactive oxygen species (ROS) and proinflammatory cytokines on calcium handling in ventricular myocytes.

Following the award of my PhD in 2009, I took a Post Doctoral position as a cardiac, cellular electrophysiologist with Professor David Eisner and Professor Andrew Trafford in the Institute of Cardiovascular Sciences, University of Manchester. Here, I helped to develop a fluorescent microscopy technique where cells are co-loaded with multiple calcium indicators. This allows the simultaneous, real-time measurement of calcium from multiple sub-cellular compartments. Using this technique, in combination with single cell patch-clamping, I contributed to the understanding of how cardiac intracellular calcium handling is regulated in health, and how this regulation becomes dysfunctional in disease states such as heart failure.

I took up my current position as a Lecturer in Biomedical Science at the University of Salford in September 2014.

Research Interests

Disease in which heart failure is implicated accounts for 160,000 annual deaths in the UK. To develop effective therapies which prevent, treat and cure heart disease, it essential that we understand the cellular mechanisms of cardiac function.

My particular focus investigates how calcium handling is regulated at the level of the individual cardiac myocyte, and, how this regulation becomes dysfunctional in various disease states. Many diseases are associated with elevations in reactive oxygen species (ROS) and proinflammatory cytokines, so I am particularly interested in how these mediators affect cardiac cellular function.

My experimental expertise lie in real-time fluorescent imaging using epifluorescent and confocal microscopy combined with electrophysiological techniques such as single cell patch-clamping.


2015 - Royal Society Early Career Research Grant - £14,762

2015 - College of Science and Technology Research Development Fund Grant - £5,545

2015 - University of Salford Research Pump Priming Grant - £4,783

2015 - University of Salford Vice Chancellors Scholarship Award - £2,000

2015 - Physiological Society Research Grant - £9,950

Key Qualifications
University Prize for Biological Sciences and Institute of Biology Award for Top Bioscience Student
Key Projects
Key Publications
Refereed Journal Articles
Meeting Abstracts
Key Presence
Conference Organisation
Honorary Posts (e.g. Visiting Appointments)
Memberships of External Bodies